Pcr Strips provide an efficient way for medical labs to conduct DNA testing. With each sample arranged in its tube, lab technicians can easily keep track of their work and ensure accurate results.

PCR tubes with attached caps also protect samples from contamination and evaporation. This is a great benefit in busy settings like urgent care clinics.

PCR tube strips allow researchers to process multiple samples simultaneously in a convenient, organized format. They also offer advantages over traditional individual tubes and caps, including contamination prevention and time savings. They are ideal for high-throughput PCR applications, as they eliminate the need to handle each individual sample and cap separately.

PCR strip tubes are made of clear or translucent polypropylene, which is thermally stable and resistant to chemicals. They are slender and cylindrical, with a capacity of 0.1 and 0.2 ml per tube. They are commonly used in molecular biology and genetics laboratories to perform PCR (polymerase chain reaction) experiments.

These PCR tubes feature durable bands between the wells that prevent them from breaking apart inadvertently. They also have large end tabs for easy opening and removal. They are labeled for orientation and fit into standard 0.2mL block thermal cyclers. The thin, consistent wall thickness optimizes heat transfer and maximizes yield.

The attached caps on these PCR tubes ensure a tight seal and reduce sample evaporation, which is common in low-throughput PCR applications. They also provide a convenient way to identify samples, as each tube has an alphabet letter engraved on the cap hinge. They come in a choice of flat caps for PCR or optically clear caps for qPCR.

Contamination is a major problem in many laboratory settings, and PCR tube strips are an excellent solution for reducing this issue. They allow users to open and close a number of tubes at the same time, which cuts down on handling and minimizes the risk of contamination. They also eliminate the need to keep track of separate caps, preventing mix-ups and sample loss.

OptiMate PCR/qPCR strip tubes are molded with the specific needs of the user in mind. They are designed to fit into thermal cyclers and accommodate various testing parameters. They are produced using premium-grade, medical-grade plastics that are formulated to avoid common contaminants, including RNase, DNase, DNA, and pyrogens. The plastics are then molded with precision to ensure that the walls have uniform thickness and consistency, which helps to guarantee accurate results.

Eppendorf Fast PCR Tube Strips

Eppendorf Fast PCR Tube Strips are made of polyethylene, which transfers heat faster than tubes made of traditional plastics. When combined with fast reagent kits and a high-performance thermal cycler, this makes the tubes ideal for speeding up PCR protocols and achieving visibly improved yield. They are also easy to open, providing a tight seal that prevents evaporation during PCR.

The tubes have an even wall thickness and smooth surface that facilitates efficient heat transfer, resulting in optimal results. The caps have a 45deg angular attachment that prevents hinges and lips from interfering with one another, so they can be opened easily while avoiding contamination or spillage. The tubes and caps are also ‘PCR clean’, meaning that they are certified free of common PCR contaminants, including DNA, DNAse, RNase and PCR inhibitors.

Unlike standard single-use PCR tubes, these strips contain a string of up to eight tubes that are linked together using a robust inter-well linkage. This makes them more rigid than individual tubes, decreasing the likelihood of spillage during handling or autoclaving. They are also designed to fit snugly into a thermal cycler, ensuring that they won’t fall over or become dislodged during the cycling process.

These PCR tubes are compatible with real-time qPCR applications and have white wells, which optimize light transmission and increase the signal intensity in fluorescent assays by eliminating interference from the thermal block. The caps are also optically clear, allowing the fluorescent signal to pass through unimpeded for enhanced sensitivity and reproducibility.

The tubes and caps are available in a range of purity grades, including Eppendorf quality, PCR clean, Biopur, Sterile and Forensic DNA Grade. Each batch of consumables is subject to rigorous inspection and testing before it can be shipped out, ensuring that customers receive high-quality products every time. To view a certificate of analysis for a particular batch of products, please enter the Lot and Batch Number in the search bar above. If you cannot find the document you need, please contact us.

DiamondLink PCR Tubes & Caps

PCR tubes and caps play an important role in ensuring accurate and reproducible results. They are in direct contact with reagents and samples and require special material properties, which can withstand temperature changes during thermal cycling, prevent condensation, provide sample protection and impart leak-proof sealing. They must also be easy to handle and offer good optical transmission. Moreover, they must be produced in a clean production workshop and undergo strict QC procedures. The Diamond PCR Tubes and Cap Strips from Eppendorf offer all of these benefits and more.

They are made from medical grade polypropylene, which is biodegradable and resistant to a wide range of chemicals. These features make them ideal for use in PCR, real-time (RT) and qPCR experiments. Moreover, their thin walls enable efficient heat transfer and reduce cycle time. They are also RNase, DNase, Human Genomic DNA, and PCR inhibitor free. Additionally, they are suitable for use with most thermal cyclers, sequencers, fragment analysers, and genotypers.

The PCR tubes have attached caps that are easy to open and close without tools, providing optimal seal to minimize evaporation. They are available with domed caps, flat caps, and optically clear strip caps. These caps can be easily labeled using a pen or barcode stickers. Moreover, they are easily identifiable due to the alphabet letters on the hinge of the cap.

Additionally, the PCR tubes have a low-profile design that ensures minimal dead space between the caps and heated lid of the thermal cycler, preventing condensation and reducing sample loss. This ensures better and more reliable results. Additionally, the PCR tubes are easy to grip and have a smooth surface, which makes them less likely to scratch or deform during handling.

To minimize potential contamination, PCR tubes should be sealed in their original packaging until they are ready for use. They should be organised on a clean 0.2 mL tube rack and labeled clearly with a marker. Afterwards, they can be stored in a clean 0.2 mL tube box or in a clearly labelled ziplock bag.

NEST PCR Tubes

PCR is an essential tool for the diagnosis of fungal diseases, as well as for determining the presence of bacterial and viral pathogens in plants. Conventional bench-top PCR systems require complex machinery, expensive reagents and trained personnel to operate. They are also difficult to bring on-field, due to their requirement of gel electrophoresis in a laboratory setting to quantify and identify the amplified DNA.

Multiplex PCR platforms allow the simultaneous detection of target sequences in a single reaction. This method is useful in reducing the number of reactions and reagents needed, as well as cutting down on sample preparation time and overall testing costs. These platforms can be used to detect a wide range of diseases and infections, including community-acquired respiratory infections such as influenza, mycoplasma and Chlamydophila; agents of gastrointestinal infection such as Campylobacter, Salmonella, Shigella, Clostridioides and Giardia; and causative agents of meningoencephalitis including Neisseria meningitidis and herpes simplex virus.

One challenge of multi-tube PCR is the contamination of the second round of amplification by off-target sequences generated by the first reaction. This is addressed by the use of nested PCR, which involves the use of two pairs of primers. The first pair binds to the flanking regions of the desired sequence, while the second pair anneals to sites within the sequence itself. This ensures that only the desired sequence is re-amplified in the second reaction, eliminating off-target sequencing products and increasing assay sensitivity.

A disadvantage of nested PCR is the need to transfer the external-primer product from the first-round reaction to the second-round internal-primer reaction, which can introduce carryover contamination and make it more difficult to quantitate the resulting amplicons. This can be overcome by physically separating the first-round and second-round reactions using a layer of wax or oil, but this can still result in contamination in the form of a non-specific amplicon.

Another way to reduce carryover is by using a molecular beacon probe, which consists of a fluorescent reporter dye and a quencher molecule that form a hairpin structure in the target DNA. The quencher molecule blocks the fluorescence of the reporter dye, which can only be detected when it is cleaved by a corresponding binding site in the PCR product.